If you have Fix Separation turned on, you will never be able to separate the parts, no matter how much load you put into the model. For best results, always turn the separation off and insert a contact between the components. And if you're interested in the fastener results, enable Account for Stiffness. Your surfaces are trying to separate, but they are bonded. A positive separation stress means you have a valid interface. Separation stress is the tensile force at the interface to the area in tension at the interface. Can you share the model?
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